Diagnosis of vaginitis in married women by microbiological and molecular methods in Tikrit City.

Abstract

The main aim of this study was identification of most common microorganisms associated with vaginitis.
This study was performed between Feb. 2013 and Juan. 2014, involving 215 married women at 15- 49 years who attending TGH and private clinic in Tikrit city; they were examined and taking high vaginal swab. For diagnosis, used Amsel's criteria, Gram staining of vaginal discharge, culture and polymerase chain reaction @CR).
Vulvovaginal candidiasis is most common 70(32.6%> woman infected with C. albicans than BV associated G. vaginalis 18(8.4%), in our distinct. Recognize Candida spp. are not always done by culture, with presence of E. coli and S. aureus were 25(11.6oh) and16(7.4o/o)  respectively. The most affected age was 2529 year (19.5%). The history of abortion and PTL in those patients was at lst and 3rd trimester of pregnancy. In this study molecular method (PCR) was used to diagnose BV associated G. vaginalis and at the sametime to diagnose Candida spp. PCR is a novel toot with excellent sensitivity and specificity especialy in fastidious IVLO as G. vaginalis. By disc diffusion method showed resistance l00o/o to metronidazole.
We conclude that Candida vaginitis is most common in our distinct. The clue cells and measurement vaginal pH are the most suitable tests of Amsels criteria for prediction of BV associated G.vaginalis. In addition, the use of metronidazole is not always benefit so we need further study of screening and use of newer local antibiotics and disinfectants.