Quantitative determination of endogenic human Pseudomonas aeruginosa exotoxin A (PEA) concentrations in serum of renalfailure patients by ELISA.

Abstract

P.aeruginosa is one of the most danger cause of morbidity and mortality in patients in nosocomial infection and renal failure. ELISA method this assay has high sensitivity and excellent specificity for detection oflruman PEA. The medical records of 64 patients (42 males and 22 females of renal failure patients ) undergoing hemodialysis at the Department of Nephrology-hemodialysis  of the Tikrit Teaching Hospital from (12 - 2012 to 12-2013 ) were retrospectively reviewed. Control of exotoxin concentration  is zero because the minimum detectable dose of human PEA concentration is typically less than 0.039ng/ml depend on sensitivity of ELIZA kit.Detectable exotoxin A in serum of 64 patients The result showin this study maximum concentration was 3.5 nglml .positive result was 37 males of 42 and 17 females of 22.and no significant exotoxin A concentration between males and females.