Effectiveness of some chemotherapeutic drugs on viability of Entamoeba histolytica trophozoites in vitro

Abstract

Amoebic dysentery is an endemic disease in Iraq. Serious steps are needed in order to decline infection ratio and to prevent any complications resulted from this disease.
The study was carried out in vitro to evaluate the efficacy of some chemical drugs on the viability of Entamoeba histolytica isolated from human in culture.
Locke egg medium was used as growth medium for isolation of Entamoeba histolytica. Best results were obtained using this medium in isolation of trophozoites. After isolation, trophozoites were tested against a variety of chemical drugs using Pandroff tube which contain Jones broth medium. Various concentrations were used from chemical drugs which included metronidazole, albendazole, and mebendazole.
Entamoeba histolyica was diagnosed in 514 stool samples (41.12%) from a total of 1250 examined sample.
Assessment of the results was done by estimation of viable trophozoites in haemocytometer chamber. The viability of trophozoites was estimated using various concentrations of (100, 50 and 25 mg/ml) of metronidazole,  albendazole and mebendazole.
Metronidazole was found to have a best inhibitory effect against Entamoeba histolytica, followed by albendazole and mebendazole which was less effective in comparison with metronidazole.