Evaluation some virulance factor of Eschirichia coli that causes diarrhea in children by using polymerase chain reaction technique in kirkuk city

Abstract

The presented study was carried out from May2015 to September 2015 in the Pediatric General Hospital in Kirkuk. A total of 102 samples from children detect some virulance factor of Escherichia coli,. The identification of these bacteria is depended on several biochemical tests including (indole test,
methyl red test, vogas proskaur test, citrate utilization test, urease test, oxidase test and motility test) and the identification was confirmed by using Analytical profile index (API 20 E system). Antibiotic Susceptibility test was done for these isolates to detect the ability of this bacteria to drug resistance.
Finally the genes that are responsible for diarrhea in E.coli isolates (eae gene for EPEC, ial gene for EIEC, It gene for ETEC,stx gene for EHEC) detected using PCR E.coli were the most common pathogen isolated from children (30isolates), and pathogenic Ecoli was 11 isolated,4 was ETEC, 4EIEC,3EPEC, and absent of EHEC.