Association between Leptin 2548 GIA gene polymorphism and serum Leptin in patients with psoriasis

Abstract

Background: Psoriasis is a disorder with genetic and immunologic background. Leptin can involve in development of psoriasis by regulation the T-helper response.
Objective: Our primary goal is to study the effect of polymorphism (G-2548A) of the leptin (LEP) gene as a risk factor for predisposition of psoriasis, and relation between the leptin serum levels and leptin 2548 gene polymorphism.
Methods: The study involved 100 patients with psoriasis and 80 healthy controls. Analysis of G-2548A polymorphism of the LEP gene was made by the PCR and restriction fragment length polymorphism technique. The relationship between LEP gene polymorphism and the clinical features
of the patients was analysed. Serum leptin levels and proportions of comorbidities in patients vs controls were compared.
Results: In controls, the GA, AA and GG frequencies were 50%, 30% and 20%, respectively, while in patients, the distribution of genotypes was 48%, 20% and 32%, respectively, with significant difference between patients and controls. There were a significant differences in the genotype distribution of Leptin -2548 cytokines
examined with respect to its serum levels in patients group. the GG genotype mean of serum level was(23.4 ± 4.1ng/ml), and GA were 20.5 ± 6.84 and AA was (16.2 ± 5.86 ng/ml).
Conclusion: LEP G-2548A polymorphism could be a predictor for higher plasma leptin and increased risk of psoriasis and could be used as a marker for psoriasis-related comorbidity risk.