Evaluation the relation of TNF-alpha in aborted women who suffer from bacterial vaginitis

Abstract

Background  The  role  of  infectious  diseases  in  recurrent  miscarriage  is  not clarified yet, but proposed an incidence of 0.5–5%. There are some candidate infectious diseases such as Listeria monocytogenes, Toxoplasma gondii, rubella, herpes simplex virus (HSV), measles, cytomegalovirus, and coxsackie viruses. Aim: This study was aimed at evaluation of the relation of TNF-α level in aborted women infected with bacterial vaginitis. 
Material and Method A cross sectional study was carried out in Kirkuk city from  the beginning of February  2018 to the end of May 2018, deep vaginal swab was collected  from 100 women with recurrent abortion and 50 pregnant women (as control group) that belonged to different geographical areas in Kirkuk city and  admitted  to Kirkuk General Hospital. The swabs samples were  cultured on blood agar and MacConkey  agar for 24 hour and secondary cultures were done for resulted culture for isolate specific bacteria. Five ml of blood was collected from each  women  enrolled  in  this  study  for  TNF-α  test  using    ELISA  technique. Results The study showed that 57% of  women  with recurrent abortion  have positive  high  vaginal  swab  (HVS)  culture comparing  with  48%  of  pregnant women (control group). The current study showed that  86% of   women had aborted in the 1st  trimester of pregnancy and the least rate of abortion was in the 3rd trimester. The study showed that 52.25% of aborted women were belonged to the age group 27-36 years  and highest rate of isolated bacteria from the HVS culture of aborted women was  S. aureus (45.61%) while  Proteus spp. was not isolated from aborted women. The study found that the highest mean of TNF-α level was denoted among women with abortion comparing with the control group (26.79±9.4 v.s 9.236±0.6 pg/ml) with highly significant relation of TNF-α level  with abortion. The study showed that the highest TNF-α mean levels was recorded among women who aborted in the 1st  trimester (28.75±8.76 pg/ml) and the lowest mean was noted among the 3rd  trimester aborted women with highly significant relation among the groups. 
Conclusions It was concluded that the there was a highly significant relation of TNF-α  level  with  abortion  specially  with  women  suffered  from  bacterial vaginosis.