A comparative study for some aspects of energy metabolism in two samples of intestinal cestodes: in house frog and domestic pigeon

Abstract

Two intestinal  cestodes  were  chosen to make  a compares,  considering  some  aspects  of energy  metabolism;   Ophiotaenia   bofonis habitat  small  intestine  of  the  house  frog  Bufo viridis  viridis, and  Cotugnea  columbae  habitat  small  intestine  of  the   domestic  pigeon Columba livia domestica.

The  present   study  reviled  that  concentration   of  macrobiomolecules    , the  energy  raw materials,   in   O.  bofonis  is  higher  than  that   of  C.  columbae.  There  were significant differences  at  p<0.05  between  the  concentration   of  total   carbohydrates,   protiens   and lipides:  (240,  181 pg/ml and  196mg/100ml,  respectevely  in the pigeon  cestode  and   105,  53 pg/ml and 133mg/100ml, respectively  in the frog cestode).

High performance liquid chromatography technique (HPLC) analysis for fatty acid elucidated that each of the two helminthes contain six types of fatty acid. O. bofonis found to have three saturated fatty acids: stearic, palmatic and myristic, 2.72%, 2.29% and 0.89% respectively and 7.91%,  respectively.  On the  other hands,  C. columbae  found to have  four  saturated and three unsaturated fatty acid: linoleic, linolienic and oliec, 73.13%, 13.07% fatty acid: stearic, palmatic, myristic and caproic, 51.43%, 45.89%, 2.38% and 0.19% respectively, and two unsaturated fatty acides: linoleic, linolienic, 0.09% and 0.06%, respectively.

Enzymatic  study  pointed  to the  significant  differences  at  p<0.05  between  the  enzymes activity in frog cestode and that of pigeon cestode. Activity of GGPDH  and LDH (cytosolic enzymes)  and  SDH  and FR  (Mitochondrial [U/L,  130 nm FAD/min/  mg   protien  and  198 nm NADH/min/mg  protien  in  O. bofonis respiretory   enzymes),  were  :7.514 U/L,  56 extract;     and    7.514    U/L,    56    IU/L,    130   nm    FAD/min/     mg       protien     and    198   nm  ADH/min/mg  protien in C. columbae extract.

The prevous resultes may reffered to a relativly higher activity of the enzymes under study, those take a part in glycolisis and energy metabolism libration in O. bofonis than that in C. columbia, in spite of that the occumulated souces of energy were more aboundant in pigeon cestode.