The Effect of Vitamin D on Biochemical Parameters among Childhood Obesity

Abstract

Background: A lack of vitamin D has been found to be a possible risk factor for a number of negative health effects.

Materials and Methods: This study examined the association among creatine kinase (CK) activity and vitamin D levels in children aged 6 to 12 who were obese and those who weren't. Between January and July 2025, sixty participants—30 obese and 30 healthy people who were matched for age and sex—were enlisted from pediatric outpatient clinics in Baghdad, Iraq. The World Health Organization's growth standards were used to measure anthropometric data, such as body mass index and waist circumference. Serum was kept at -20°C until analysis after venous blood samples were drawn and handled in a sterile manner. Enzymatic colorimetry was used to evaluate CK enzyme activity, Vitamin D concentrations were measured by ELISA. Statistical analyses were performed in SPSS using independent-samples t-tests to compare obese and control groups and Pearson’s correlation to evaluate associations, with significance set at p < 0.05.

Results: Compared with controls, obese children had significantly lower vitamin D levels and significantly higher creatine kinase (CK) activity; waist circumference and body mass index were also significantly greater in the obese group, consistent with metabolic alterations associated with obesity.