Measurement of Osteocalcin as a bone turnover marker in women before & after menopause in Tikrit city

Abstract

Osteocalcin is a small protein (49 amino acids) mainly synthesized by mature osteoblasts, remains the most specific marker of osteoblastic activity. Serum osteocalcin values are higher in children than in adults, rising during puberty and decreasing to adult leveis. Most studies show a higher concentration of osteocalcin in adult males than in adult females, but there are no data available regarding the levels in osteocalcin during human ageing. The aim of the study is to measure serum osteocalcin as a bone turnover marker in women before & after menopause in relation to steroid hormones in Tikrit city. The study included 95 female
subjects who attended to Dejla hospital for measurement of bone mineral. Fifty subjects from the total 95 subjects are postmenopausal women. Subjects distributed according age into four subgroups ;- Fourteen women in group 1 aged less than forty years, 27 subjects in group 2 aged 40-49 years, 27 subjects in group 3 aged 50-59 years, & 27 subjects in group 4 aged 60 years & above. Serum osteoclacin was measured by the lectrochemiluminescence immunoassay (ECLIA) is intended by use on Elecsys and Cobas (e411 (Roch Device) immunoassay analyzers. There is no significant difference in regard to serum osteocalcin concentration between group aged 40-49 years (15.63 $ 6.54 ng/ml) as compare to age group less than 40 years (16.229 $ 4.7 ng/ml). However, there is significant increase in serum osteocalcin concentration in group aged 50-59 years (20.95 ‡ 13.6 ng/ml) as compare to age
group less than 40 years (16.229 # 4.7 ng/ml). Also, there is significant increase in serum osteocalcin concentration in group aged 60-69 years (18.06 $ 7.32 ng/ml) as compare to age group less than 40 years (16.229 $ 4.7 ng/ml). There are fifty postmenopausal women, they have a significant increase in serum osteocalcin concentration (23.32 ‡ 14.1 ng/ml), as compare to age group less than 40 years (16.229 $ 4.7 ng/ml) & group 40-49 years (15.63 ‡ 6.54 ng/ml). The present study shows that there is significant increase in serum osteocalcin concentration associated with increase in age of women.