Myocardium and Coronary artery architectural changes in ovariectomized rabbit and its relation with serum estrogen level

Abstract

Forty eight sexually matured adult female rabbits were divided into 6 groups, control, A, B, C, D and E. The experimental groups were overictomized  and  scarified  at  the  time  interval  of  zero,  7,  14, 30,60and90 days respectively. Blood samples were collected twice, one before  the  operation  and  the  second  one  before  scarifying.  Blood samples were centrifuged and then, serum was obtained and tested for estrogen. Also cardiac tissue and coronary arteries were prepared and stained with hematoxylin and eosin (H&E) method. And slides were then examined. The results showed statistically significant difference in estrogen level, and was declining with time after bilateral overictomy , and was ( 29.45 ± 0.65  ) (pg/ml)in control, while it was lower in the other groups  the difference finally group E  showed widely spaced cardiac fibers with fat deposition replacing the losted cardiomyocytes with congested thick walled blood vessels. 
  The  coronary  artery  in  the  control  and  group  A  showed  normal endothelial cell distribution without degenerative changes with normal wall thickness and lumen. The endothelial cells in group B showed minor changes, the wall thickness increased. No major changes in the lumen, more epithelial cell distribution and irregular outline in group C when compared to group Atheroma formation and clear atherosclerotic changes.