Effect of punica granatum extract on growth of some serotypes of Streptococcus pyogenes isolated from acute and chronic cases of Tonsillitis in Children

Abstract

Unregulated  and  frequent  antibiotic  use  may  cause  microbial resistance  in  dangerous  organisms.  Thus,  innovative  synthetic  and natural antibacterial compounds are necessary. In this perspective, plants provide  most  natural  compounds.  This  study  aimed  to  isolation  and identification of Streptococcus pyogenes from acute and chronic cases and investigate the antibacterial properties of pomegranate peel extracts. The study included the collection of 215 samples (15 control and 200 patients from acute and chronic tonsillitis children), their age from(2 to 12 years) in kirkuk city during the period from (15-10-2023 to 1-2-2024) 140 (70%) showed no growth. A total of 200 clinical samples were obtained from patients. All samples were grown on blood agar and Azide blood agar and incubated aerobically at 37 °C for 24 hours. About 60 (30%) [40 chronic (20%) and 20 acute (10%)] of the samples showed positive  for  Strep.  pyogenes growth. Three  types  of  extracts  of  P. granatum pomegranate peels were used (acetone, ethanolic and aqueous extract). The aceton extract was more effective than ethanolic and watery ,  which  inhibition  zone  were  (29.3,  26.5,  14.55)mm.  This  study determined  that  the  extracts  from  pomegranate  peels  had  potent antibacterial  properties  against  bacteria  and  have  the  potential  to  be utilized as medicinal treatment for human diseases caused by bacteria that are resistant to several drugs. The aceton extracts of pomegranate have higher antibacterial activity compared to the ethanolic and aqueous extracts.