Evaluation of Serum Osteopontin and Growth Differential Factor-15 in Patients with Heart Failure

Abstract

Background:  Heart  failure  (H.F)  is  a  condition  in  which  the  heart cannot supply the body's tissues with enough amount of blood. Certain conditions such as narrowing arteries in heart (coronary heart disease) or hypertension  gradually  make  heart  weak  or  stiff  to  fill  and  pump efficiently.  Osteopontin  (OPN)  is  a  glycoprotein  expressed  in  many types of cells including bone, immune, endothelial cells, and others. OPN  has  an  important  role  in  mineralization  and  bone  resorption. Growth differential factor-15 (GDF-15) is a member of transforming growth  factor  β  (TGFβ)  cytokine  superfamily,  GDF-15  is  highly expressed in cardiomyocyte, adipocyte, macrophage, and other cells. Aim: to evaluate levels of OPN and GDF-15  in patients with heart failure  
Patients and methods: This study was achieved from August 2020 to October 2020, it was conducted on 22 patients with CVD who attended to cardio care unit (CCU) in Al-Ramadi teaching hospital in period from August to October 2020. The results were compared with 30 healthy control subjects. About four milliliters of venous blood were collecting in gel tube from patients and controls to estimate OPN and GDF-15 by ELISA  method.  Furthermore,  fasting  blood  sugar  (FBS)  and  total cholesterol (CHO) were measured for patients and control groups.  Results: the level of OPN was significantly high in H.F patients (51.18± 23.57 ng/ml) compared to control group (1.07±0.41 ng/ml) furthermore FBS  and  CHO  mean  concentrations  were  significantly  high  in  H.F. patients  (195.46±89.16  mg/dl),  (153.38±45.14  mg/dl)  respectively, compared   with   control   group   (FBS   97.08±13.84   mg/dl),   (CHO 112.18±10.64 mg/dl).   
Conclusion: the study’s results showed that a significant increase in level of OPN and GDF-15 as a biomarker for heart failure patients and there  was  a correlation  between both  OPN  and GDF-15  with  blood glucose concentration, also hyperlipidemia was present in H.F. patients.