Molecular Detection of Adenovirus Genotype 3 in Adult Patients with Respiratory Infection

Abstract

Background  The  DNA  virus  known  as  adenovirus  was  initially identified in the 1950s in soldiers who were suffering from pneumonia that  was  unusual.  There  are  now  seven  species  (A–G)  of  human adenovirus (HAdV) with over 100 genotypes. Disease types and severity vary depending on the genotype of infection by adenovirus. In children as well as adults, human adenovirus type three is a significant respiratory infection that spreads and causes complications.
The  main  objective  of  this  study:  was  to  identify  adenoviruses genotype 3 by real time PCR from plasma sample of adult patients with respiratory infection. This research was carried out at Kirkuk Teaching Hospital/Azadi  Teaching  Hospital  and  Gynecology,  Pediatrics  and Woman Hospital in Kirkuk City between the first of November 2024, and the fifth of February 2025. One hundred patients' plasma was tested for adenovirus genotype 3 by using Real Time PCR Technique, there were 42 (42%) female patients and 58 (58%) male patients. The findings indicated that 76 patients (76%) had negative results and 24 patients (24%) had positive result. Adenovirus type-3 DNA detection did not significantly differ between males and females (52.26% and 47.74%, respectively;   p>0.05).   The   investigation   revealed   an   insufficient correlation between location, age and gender with positive results.