Antimicrobial activity of two plant extracts against bacterial pathogens isolated from patient with urinary tract infection

Abstract

This study included isolation and diagnosing some bacterial species that cause urinary tract infections and testing the effect of aqueous and alcoholic extracts of garlic and ginger fruits on it . A number of urine samples (200) were collected and taken from patients and patients at Azadi Hospital, for the period 18/11/2021 to 10/3/2022, and included males and females for ages between (15-60) years, with a ratio of ( 70%fmales and (30%) males After planting the samples on blood agar, MacConkey agar, and Mannitol salt agar media, (150) samples of them gave bacterial growth when cultured, with a rate of (75%), as it gave two types of growth, negative for gram stain (73.3%), and  positivefo  Gram  stain  (26.7%),  while  the  other  50  samples  did  not  give  any bacterial growth. The infection rate was higher among females (70%), while males (30%), and the highest infection rate was recorded in the age group (15-30) years in both sexes. Bacterial isolates were isolated and diagnosed based on their morphological characteristics  an111d  biochemical  tests.  The  following  species  appeared:    Escherichia  coli 45  isolates  (30%),  Klebsiella    pneumonia 25  isolates  (16.7%), Staphylococcus  aureus  40  isolates  (26.6%),      Pseudomonas  aeruginosa  15  isolate (10%),  Proteus    mirabilis  25  isolate  (16.7%).  The  effectiveness  of  aqueous  and alcoholic extracts was tested at concentrations (25%), (50%) and (100%), where the alcoholic  extract  was  more  efficient  compared  to  the  aqueous  extract  and  the concentration was (100%) is the most effective concentration on bacterial isolates and the least at the extract at a concentration of (25%) for the aqueous or alcoholic extract..