Design and Analysis on platelet-rich plasma for Tendon healing in Rats

Abstract

To  treat  tendon  injuries,  platelet-rich  plasma  (PRP)  is  widely utilized in orthopedic surgery and sports medicine. Tendinopathy, a condition of the tendons, can be treated using PRP. The purpose of this study is to examine the applicability of the combination of three different cell types and PRP on tenocyte-conditioned medium (CM)  to  investigate  the  potential  effect  on  promoting  tendon healing.  Tenocytes,  bone  marrow  mesenchymal  stem  cells  and dermal  fibroblasts  were  cultured  in  a  three-dimensional  PRP hydrogel with or without CM, and the proliferation and migration of  the  cells  were  observed.  Through  reverse  transcription quantitative  polymerase  chain  reaction  and  enzyme-linked immunosorbent assay, the CM derived from tendon was applied to mesenchymal  stem  cells,  tendons,  and  skin  fibroblasts, respectively, to determine its effect on matrix formation phenotype and  inflammatory  protein secretion.  Tendon  cell CM  combined with PRP stimulated tendon formation of mesenchymal stem cells and  skin  fibroblasts  and  reduced  the  secretion  of  inflammatory proteins. In conclusion modifying the target tissue with PRP before cell  implantation  can  optimize  the  effect  of  cell  therapy.  Skin fibroblasts and bone marrow mesenchymal stem cells combined with PRP can be used to regenerate tendons.